%0 Journal Article %T Contribution of the glycolytic flux and hypoxia adaptation to efficient biofilm formation by Candida albicans. %+ Biologie et Pathogénicité fongiques (BPF) %+ Hôpital Côte de Nacre [CHU Caen] %+ Imagerie Dynamique (Plate-Forme) (PFID) %A Bonhomme, Julie %A Chauvel, Murielle %A Goyard, Sophie %A Roux, Pascal %A Rossignol, Tristan %A d'Enfert, Christophe %Z Thanks are due to Malcolm Whiteway and colleagues for pointing the hypoxia-dependent morphogenesis phenotype ofthe Dtye7 mutant. We are grateful to Caroline Proux and Jean-Yves Coppée at the PF2 Platform of Pasteur GénopoleIle-de-France for help with micro-array experiments. We are also grateful to past and present members of the FungalBiology and Pathogenicity Unit, Guilhem Janbon and Ismaïl Iraqui for continued interest and suggestions during the courseof this study. J.B. was the recipient of a fellowship of the Fondation pour la Recherche Médicale (DEA20080713005).T.R. was the recipient of a postdoctoral fellowship in the framework of the NPARI consortium (LSHE-CT-2006-037692). This work was supported by grants from Institut Pasteur (PTR50), the Ministère de la Recherche et de laTechnologie (PRFMMIP, ‘Réseau Infections Fongiques’) and the European Commission (QLK2-2000-00795, MRTN-CT-2003-504148, PITN-GA-2008-214004). %< avec comité de lecture %@ 0950-382X %J Molecular Microbiology %I Wiley %V 80 %N 4 %P 995-1013 %8 2011-05-02 %D 2011 %R 10.1111/j.1365-2958.2011.07626.x %M 21414038 %K GENE-EXPRESSION %K FILAMENTOUS GROWTH %K PYRUVATE-KINASE %K YEAST %K DISRUPTION %K MECHANISMS %K TRANSCRIPTIONAL RESPONSE %K SACCHAROMYCES-CEREVISIAE %K PROTEIN %K CELL %Z Life Sciences [q-bio]/Microbiology and Parasitology/MycologyJournal articles %X The fungal pathogen Candida albicans forms therapeutically challenging biofilms on biomedical implants. Using a transcript profiling approach genes whose expression is favoured upon biofilm growth compared with planktonic growth have been previously identified. Knock-out mutants for 38 of these genes were constructed, six of which showed a specific defect in biofilm formation. Among these genes, TYE7 that encodes a transcriptional activator of glycolytic genes in planktonic and biofilm growth conditions was identified as being required for the cohesiveness of biofilms. Biofilms formed by the tye7Δ knock-out mutant showed a hyperfilamentous morphology, and growth of this mutant on solid medium under hypoxia was also associated with the production of hyphae. Similar to TYE7 inactivation, inhibition of glycolysis or ATP synthesis using oxalate or an uncoupler, respectively, triggered morphogenesis when a wild-type strain was grown under hypoxia. These treatments also induced the formation of weakly cohesive, hyper-filamentous biofilms by a wild-type strain. Our data indicate that a hypoxic environment is generated within C. albicans biofilms and that continued biofilm development requires a Tye7p-dependent upregulation of glycolytic genes necessary to adapt to hypoxia and prevent uncontrolled hyphal formation. Thus, adaptation to hypoxia is an integral component of biofilm formation in C. albicans. %G English %2 https://pasteur.hal.science/pasteur-01524629/document %2 https://pasteur.hal.science/pasteur-01524629/file/Bonhomme_et_al-2011-Molecular_Microbiology.pdf %L pasteur-01524629 %U https://pasteur.hal.science/pasteur-01524629 %~ PASTEUR %~ INRA %~ OPENAIRE %~ COMUE-NORMANDIE %~ AGREENIUM %~ INRAE %~ FRM