%0 Journal Article %T Pleiotropic effects of the vacuolar ABC transporter MLT1 of Candida albicans on cell function and virulence. %+ Jawaharlal Nehru University (JNU) %+ Leibniz Institute for Natural Product Research and Infection Biology (Hans Knoell Institute) %+ University of Lucknow %+ Stony Brook University [SUNY] (SBU) %+ Université de Lausanne = University of Lausanne (UNIL) %+ University of Wisconsin-Madison %+ Rutgers New Jersey Medical School (NJMS) %+ Centre for DNA Fingerprinting and Diagnostics [Hyderabad] (CDFD) %+ Biologie et Pathogénicité fongiques (BPF) %+ Amity University %+ Technische Universität Darmstadt, Fachbereich Material und Geowissenschaften, Darmstadt, Germany %A Khandelwal, Nitesh Kumar %A Kaemmer, Philipp %A Forster, T. M. %A Singh, Ashutosh %A Coste, Alix T %A Andes, R. %A Hube, Bernhard %A Sanglard, Dominique %A Chauhan, Neeraj %A Kaur, Rupinder %A d'Enfert, Christophe %A Mondal, Alok Kumar %A Prasad, Rajendra %Z The work has been supported, in part, by the Department of Biotechnology, Ministry of Science and Technology, Government of India [grant numbers BT/01/CEIB/10/III/02, BT/PR7392/MED/29/652/2012 and BT/PR14879/BRB10/885/2010 (to R.P.)]. N.K.K. acknowledges the University Grants Commission, India, for a senior research fellowship award. Financial Support from the Indo–Swiss Joint Research Programme [grant number 122 917 (to D.S. and R.P.)], for supporting N.K.K.’s visit to CHUV, Lausanne, is acknowledged. %< avec comité de lecture %@ 0264-6021 %J Biochemical Journal %Y David Carling %I Portland Press %V 473 %N 11 %P 1537-52 %8 2016-06-01 %D 2016 %R 10.1042/BCJ20160024 %M 27026051 %K MLT1 %K phosphotidylcholine %K virulence %K ABC transporter %K Candida albicans %Z Life Sciences [q-bio]/Microbiology and Parasitology/MycologyJournal articles %X Among the several mechanisms that contribute to MDR (multidrug resistance), the overexpression of drug-efflux pumps belonging to the ABC (ATP-binding cassette) superfamily is the most frequent cause of resistance to antifungal agents. The multidrug transporter proteins Cdr1p and Cdr2p of the ABCG subfamily are major players in the development of MDR in Candida albicans Because several genes coding for ABC proteins exist in the genome of C. albicans, but only Cdr1p and Cdr2p have established roles in MDR, it is implicit that the other members of the ABC family also have alternative physiological roles. The present study focuses on an ABC transporter of C. albicans, Mlt1p, which is localized in the vacuolar membrane and specifically transports PC (phosphatidylcholine) into the vacuolar lumen. Transcriptional profiling of the mlt1∆/∆ mutant revealed a down-regulation of the genes involved in endocytosis, oxidoreductase activity, virulence and hyphal development. High-throughput MS-based lipidome analysis revealed that the Mlt1p levels affect lipid homoeostasis and thus lead to a plethora of physiological perturbations. These include a delay in endocytosis, inefficient sequestering of reactive oxygen species (ROS), defects in hyphal development and attenuated virulence. The present study is an emerging example where new and unconventional roles of an ABC transporter are being identified. %G English %2 https://pasteur.hal.science/pasteur-01518013/document %2 https://pasteur.hal.science/pasteur-01518013/file/BCJ20160024.full.pdf %L pasteur-01518013 %U https://pasteur.hal.science/pasteur-01518013 %~ PASTEUR %~ INRA %~ AGREENIUM %~ INRAE