%0 Journal Article %T Pf155/RESA protein influences the dynamic microcirculatory behavior of ring-stage Plasmodium falciparum infected red blood cells. %+ Massachusetts Institute of Technology (MIT) %+ Korea Advanced Institute of Science and Technology (KAIST) %+ Immunologie moléculaire des parasites %A Diez-Silva, Monica %A Park, Yongkeun %A Huang, Sha %A Bow, Hansen %A Mercereau-Puijalon, Odile %A Deplaine, Guillaume %A Lavazec, Catherine %A Perrot, Sylvie %A Bonnefoy, Serge %A Feld, Michael S %A Han, Jongyoon %A Dao, Ming %A Suresh, Subra %Z M.D-S, MD, and SS acknowledge support for this work from the Interdisciplinary Research Group on Infectious Diseases, which is funded by the Singapore-MIT Alliance for Research and Technology (SMART) Center. YKP was funded by KAIST, KAIST Institute for Optical Science and Technology, National Research Foundation (NRF-2012R1A1A1009082) and the Korean Ministry of Education, Science and Technology (MEST) grant No. 2009-0087691 (BRL). YKP acknowledges support from POSCO TJ Park Fellowship. The authors also acknowledge the support of the SMART BioSyM IRG, the National Institutes of Health (NIH) Grant R01HL094270, and the French Agence Nationale de la Recherche (ANR), under grant MIE (ANR-08-MIE-031) and the support of the National Center for Research Resources of the National Institutes of Health (9P41-EB015871-26A1). GD was funded by grants from the Délégation Générale à l’Armement (fellowship 05 60 00 032), F. Lacoste (Fondation Ackerman - Fondation de France) and the Région Ile de France. %< avec comité de lecture %@ 2045-2322 %J Scientific Reports %I Nature Publishing Group %V 2 %P 614 %8 2012 %D 2012 %R 10.1038/srep00614 %M 22937223 %Z Life Sciences [q-bio]/Microbiology and ParasitologyJournal articles %X Proteins exported by Plasmodium falciparum to the red blood cell (RBC) membrane modify the structural properties of the parasitized RBC (Pf-RBC). Although quasi-static single cell assays show reduced ring-stage Pf-RBCs deformability, the parameters influencing their microcirculatory behavior remain unexplored. Here, we study the dynamic properties of ring-stage Pf-RBCs and the role of the parasite protein Pf155/Ring-Infected Erythrocyte Surface Antigen (RESA). Diffraction phase microscopy revealed RESA-driven decreased Pf-RBCs membrane fluctuations. Microfluidic experiments showed a RESA-dependent reduction in the Pf-RBCs transit velocity, which was potentiated at febrile temperature. In a microspheres filtration system, incubation at febrile temperature impaired traversal of RESA-expressing Pf-RBCs. These results show that RESA influences ring-stage Pf-RBCs microcirculation, an effect that is fever-enhanced. This is the first identification of a parasite factor influencing the dynamic circulation of young asexual Pf-RBCs in physiologically relevant conditions, offering novel possibilities for interventions to reduce parasite survival and pathogenesis in its human host. %G English %2 https://pasteur.hal.science/pasteur-01472091/document %2 https://pasteur.hal.science/pasteur-01472091/file/srep00614.pdf %L pasteur-01472091 %U https://pasteur.hal.science/pasteur-01472091 %~ PASTEUR %~ CNRS %~ ANR