%0 Journal Article %T Comparative Analysis Between Flaviviruses Reveals Specific Neural Stem Cell Tropism for Zika Virus in the Mouse Developing Neocortex. %+ Compartimentation et dynamique cellulaires (CDC) %+ Environnement et Risques infectieux - Environment and Infectious Risks (ERI) %A Brault, Jean-Baptiste %A Khou, Cécile %A Basset, Justine %A Coquand, Laure %A Fraisier, Vincent %A Frenkiel, Marie-Pascale %A Goud, Bruno %A Manuguerra, Jean-Claude %A Pardigon, Nathalie %A Baffet, Alexandre D %Z The Baffet and Goud labs are part of the Labex CelTisPhyBio (11-LBX-0038) and the Idex Paris Sciences et Lettres (ANR-10-IDEX-0001-02 PSL). Seventh Framework Programme (FP7) provided funding under grant number 278433-PREDEMICS to NP, JB and MPF. Ministry of Defence/Direction Générale de l'Armement (DGA) provided funding to CK. This work was supported by the European Research Council advanced grants (project 339847 ‘MYODYN’ to B.G.). %< avec comité de lecture %@ 2352-3964 %J EBioMedicine %I Elsevier %V 10 %P 71-6 %8 2016-08 %D 2016 %R 10.1016/j.ebiom.2016.07.018 %M 27453325 %K Zika virus %K Flavivirus %K mouse neocortex %K neural stem cell %Z Life Sciences [q-bio]/Microbiology and Parasitology/Virology %Z Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/NeurobiologyJournal articles %X The recent Zika outbreak in South America and French Polynesia was associated with an epidemic of microcephaly, a disease characterized by a reduced size of the cerebral cortex. Other members of the Flavivirus genus, including West Nile virus (WNV), can cause encephalitis but were not demonstrated to cause microcephaly. It remains unclear whether Zika virus (ZIKV) and other flaviviruses may infect different cell populations in the developing neocortex and lead to distinct developmental defects. Here, we describe an assay to infect mouse E15 embryonic brain slices with ZIKV, WNV and dengue virus serotype 4 (DENV-4). We show that this tissue is able to support viral replication of ZIKV and WNV, but not DENV-4. Cell fate analysis reveals a remarkable tropism of ZIKV infection for neural stem cells. Closely related WNV displays a very different tropism of infection, with a bias towards neurons. We further show that ZIKV infection, but not WNV infection, impairs cell cycle progression of neural stem cells. Both viruses inhibited apoptosis at early stages of infection. This work establishes a powerful comparative approach to identify ZIKV-specific alterations in the developing neocortex and reveals specific preferential infection of neural stem cells by ZIKV. %G English %2 https://pasteur.hal.science/pasteur-01441052/document %2 https://pasteur.hal.science/pasteur-01441052/file/1-s2.0-S2352396416303231-main.pdf %L pasteur-01441052 %U https://pasteur.hal.science/pasteur-01441052 %~ PASTEUR %~ UPMC %~ CNRS %~ OPENAIRE %~ FNCLCC %~ CURIE %~ UMR144 %~ PSL %~ UPMC_POLE_4 %~ SORBONNE-UNIVERSITE %~ SU-SCIENCES %~ INSTITUT-CURIE-PSL %~ SU-TI %~ ANR %~ ALLIANCE-SU %~ TEST3-HALCNRS