%0 Journal Article %T Rolling Circle Amplification with Chemically Modified Nucleoside Triphosphates %+ Chimie bioorganique des acides nucléiques %+ McGill University = Université McGill [Montréal, Canada] %A Hollenstein, Marcel %A Damha, Masad, J %< avec comité de lecture %J Current Protocols in Nucleic Acid Chemistry %V 7 %8 2016-12 %D 2016 %R 10.1002/cpnc.17 %M 27911492 %Z Chemical SciencesJournal articles %X Modified nucleoside triphosphates (dN*TPs) represent facile and versatile precursorsfor the introduction of chemical diversity into nucleic acids. WhiledN*TPs have been utilized in a plethora of practical applications, very littleattention has been devoted to the assessment of their compatibility with isothermalamplification strategies. In this context, rolling circle amplification (RCA)is a wide-spread enzymatic replication method in which small single-strandedDNA (ssDNA) circles serve as templates in primer extension reactions yieldingvery long, ssDNA products. RCA is a pivotal tool for the generation of biosensorand diagnostic devices and is currently evaluated for its usefulness to createnovel drug delivery systems. This unit describes the experimental proceduresfor the synthesis of modified RCA products using dN*TPs bearing chemicalalterations at any possible location of the nucleosidic scaffold. Two ligationmethods are presented for the generation of the DNA nanocircles that serve astemplates for RCA, followed by a description of the RCA method itself and anassessment of the nuclease resistance of the ensuing products. %G English %2 https://pasteur.hal.science/pasteur-01426352/document %2 https://pasteur.hal.science/pasteur-01426352/file/CurrrProt2016.pdf %L pasteur-01426352 %U https://pasteur.hal.science/pasteur-01426352 %~ PASTEUR