%0 Journal Article %T Evidence for a dual role of actin in regulating chromosome organization and dynamics in yeast. %+ Régulation spatiale des Génomes - Spatial Regulation of Genomes %+ Pathologie cellulaire : aspects moléculaires et viraux / Pathologie et Virologie Moléculaire %+ Imagerie et Modélisation %A Spichal, Maya %A Brion, Alice %A Herbert, Sébastien %A Cournac, Axel %A Marbouty, Martial %A Zimmer, Christophe %A Koszul, Romain %A Fabre, Emmanuelle %Z This research was supported by funding to R.K. from the European Research Council under the 7th Framework Program [grant numbers FP7/2007-2013 and ERC grant agreement number 260822]; and to E.F. by Agence Nationale de la Recherche [grant numbers ANR-13-BSV8-0013-01 and ANR-13-BSV3-0012-03]; Agence pour la Recherche contre le Cancer (ARC) [grant number FI20121205474]; and Labex Who am I [grant number EE-2013]. C.Z. acknowledges funding from the Institut Pasteur and Fondation pour la Recherche Médicale (FRM). M.M. was supported by a fellowship from ARC [grant number 20100600373]; M.S. by a fellowship from Université Pierre et Marie Curie (UPMC) and the Ligue Nationale Contre le Cancer (LNCC), and S.H. was supported by the FRM. %< avec comité de lecture %@ 0021-9533 %J Journal of Cell Science %I Company of Biologists %V 129 %N 4 %P 681-92 %8 2016-02-15 %D 2016 %R 10.1242/jcs.175745 %M 26763908 %K Actin %K Chromosome %K Dynamics %Z Life Sciences [q-bio]Journal articles %X Eukaryotic chromosomes undergo movements that are involved in the regulation of functional processes such as DNA repair. To better understand the origin of these movements, we used fluorescence microscopy, image analysis and chromosome conformation capture to quantify the actin contribution to chromosome movements and interactions in budding yeast. We show that both the cytoskeletal and nuclear actin drive local chromosome movements, independently of Csm4, a putative LINC protein. Inhibition of actin polymerization reduces subtelomere dynamics, resulting in more confined territories and enrichment in subtelomeric contacts. Artificial tethering of actin to nuclear pores increased both nuclear pore complex (NPC) and subtelomere motion. Chromosome loci that were positioned away from telomeres exhibited reduced motion in the presence of an actin polymerization inhibitor but were unaffected by the lack of Csm4. We further show that actin was required for locus mobility that was induced by targeting the chromatin-remodeling protein Ino80. Correlated with this, DNA repair by homologous recombination was less efficient. Overall, interphase chromosome dynamics are modulated by the additive effects of cytoskeletal actin through forces mediated by the nuclear envelope and nuclear actin, probably through the function of actin in chromatin-remodeling complexes. %G English %2 https://pasteur.hal.science/pasteur-01419905/document %2 https://pasteur.hal.science/pasteur-01419905/file/681full.pdf %L pasteur-01419905 %U https://pasteur.hal.science/pasteur-01419905 %~ INSERM %~ PASTEUR %~ UNIV-PARIS7 %~ CNRS %~ APHP %~ OPENAIRE %~ USPC %~ UNIV-PARIS %~ ANR %~ FRM %~ SPATIAL-REGULATION-GENOMES %~ UMR3525