%0 Journal Article %T Escherichia coli K-12 possesses multiple cryptic but functional chaperone-usher fimbriae with distinct surface specificities. %+ Génétique des Biofilms %+ Microscopie électronique (Plate-forme) %A Korea, Charalampia-Georgia %A Badouraly, Reana %A Prévost, Marie-Christine %A Ghigo, Jean-Marc %A Beloin, Christophe %Z C.G.K. is supported by a MENESR (Ministère Français de l'Éducation Nationale, de l'Enseignement Supérieur et de la Recherche) fellowship. C.B and J-M.G. are supported by the Institut Pasteur, CNRS URA 2172, the Network of Excellence EuroPathoGenomics (LSHB-CT-2005-512061), the ERA-NET Pathogenomics (ANR-06-PATHO-004-01) and Fondation BNP PARIBAS grants. %< avec comité de lecture %@ 1462-2912 %J Environmental Microbiology %I Society for Applied Microbiology and Wiley-Blackwell %V 12 %N 7 %P 1957--1977 %8 2010-07 %D 2010 %R 10.1111/j.1462-2920.2010.02202.x %M 20345943 %K Adhesins %K Bacterial/*genetics/metabolism %K Bacterial Adhesion %K beta-Galactosidase/genetics/metabolism %K Cell Line %K Epithelial Cells/microbiology %K Escherichia coli K12/*chemistry %K Escherichia coli Proteins/*genetics/metabolism %K Fimbriae %K Gene Deletion %K Gene Expression Profiling %K Gene Expression Regulation %K Bacterial %K Gene Order %K Genes %K Reporter %K Humans %K Microscopy %K Electron %K Transmission %K Mutagenesis %K Insertional %K Operon %Z Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyJournal articles %X Commensal and pathogenic Escherichia coli adherence to host and environmental surfaces is mediated by a variety of adhesins. Although extensively studied as a model bacterium, 34% of the genes in the E. coli K-12 genome have no known function. We hypothesized that some of them may correspond to functional adhesins. We characterized E. coli K-12 ycb, ybg, yfc, yad, yra, sfm and yeh operons, which display sequence and organizational homologies to type 1 fimbriae exported by the chaperone/usher pathway. We showed that, although these operons are poorly expressed under laboratory conditions, six of them are nevertheless functional when expressed, and promote adhesion to abiotic and/or epithelial cell surfaces. While the studied fimbriae display different binding specificities, we obtained evidence of synergy/interference with other adhesins such as Ag43 or type 1 fimbriae. We showed that their expression is under the negative control of %G English %L pasteur-01393508 %U https://pasteur.hal.science/pasteur-01393508 %~ PASTEUR %~ CNRS %~ OPENAIRE %~ ANR