%0 Journal Article %T ISG15 counteracts Listeria monocytogenes infection. %+ Interactions Bactéries-Cellules (UIBC) %+ Institut National de la Santé et de la Recherche Médicale (INSERM) %+ Institut National de la Recherche Agronomique (INRA) %+ MICrobiologie de l'ALImentation au Service de la Santé (MICALIS) %+ Cellule Pasteur %+ Molecular Genetics Group %A Radoshevich, Lilliana %A Impens, Francis %A Ribet, David %A Quereda, Juan J %A Nam Tham, To %A Nahori, Marie-Anne %A Bierne, Hélène %A Dussurget, Olivier %A Pizarro-Cerdá, Javier %A Knobeloch, Klaus-Peter %A Cossart, Pascale %Z Howard Hughes Medical Institute (HHMI), Senior International Research Fellow - Institut Pasteur, Bourse Roux - Institut national de la santé et de la recherche médicale, U604 - Institut national de la recherche agronomique, USC2020 - Agence Nationale de la Recherche, LISTRESS, PROANTILIS - Agence Nationale de la Recherche, Laboratoire d'Excellence (ANR-10-LABX-62-IBEID) - European Research Council (ERC), 233348 MODELIST - Human Frontier Science Program (HFSP), Long Term Fellow - European Molecular Biology Organization (EMBO), Long Term Fellow - Fondation Le Roch les Mousquetaires - Louis-Jeantet Foundation %< avec comité de lecture %J eLife %I eLife Sciences Publication %V 4 %P 4:e06848 %8 2015-08-11 %D 2015 %R 10.7554/eLife.06848 %M 26259872 %K Infectious Disease %K Listeria monocytogenes %K Innate Immunity %K Endoplasmic reticulum %K ISG15 %K Infectious Disease %Z Life Sciences [q-bio]/Cellular Biology %Z Life Sciences [q-bio]/Microbiology and ParasitologyJournal articles %X ISG15 is an interferon-stimulated, linear di-ubiquitin-like protein, with anti-viral activity. The role of ISG15 during bacterial infection remains elusive. We show that ISG15 expression in nonphagocytic cells is dramatically induced upon Listeria infection. Surprisingly this induction can be type I interferon independent and depends on the cytosolic surveillance pathway, which senses bacterial DNA and signals through STING, TBK1, IRF3 and IRF7. Most importantly, we observed that ISG15 expression restricts Listeria infection in vitro and in vivo. We made use of stable isotope labeling in tissue culture (SILAC) to identify ISGylated proteins that could be responsible for the protective effect. Strikingly, infection or overexpression of ISG15 leads to ISGylation of ER and Golgi proteins, which correlates with increased secretion of cytokines known to counteract infection. Together, our data reveal a previously uncharacterized ISG15-dependent restriction of Listeria infection, reinforcing the view that ISG15 is a key component of the innate immune response. %G English %2 https://pasteur.hal.science/pasteur-01185203/document %2 https://pasteur.hal.science/pasteur-01185203/file/ELife_Radoshevich.pdf %L pasteur-01185203 %U https://pasteur.hal.science/pasteur-01185203 %~ PASTEUR %~ UNIV-PARIS7 %~ AGROPARISTECH %~ INRA %~ OPENAIRE %~ UNIV-PARIS-SACLAY %~ AGREENIUM %~ AGROPARISTECH-SACLAY %~ INRA-SACLAY %~ INRAE %~ UNIV-PARIS %~ ANR %~ GS-BIOSPHERA %~ GS-HEALTH-DRUG-SCIENCES %~ MICALIS