%0 Journal Article %T N-Glycosylation instead of cholesterol mediates oligomerization and apical sorting of GPI-APs in FRT cells. %+ Trafic membranaire et Pathogénèse %+ Dipartimento di Biologia e Patologia Cellulare e Moleculare %+ Departamento de Inmunologia Clinica y Reumatologia %A Imjeti, Naga Salaija %A Lebreton, Stéphanie %A Paladino, Simona %A de La Fuente, Erwin %A Gonzalez, Alfonso %A Zurzolo, Chiara %Z The European Union FP7 (Grant 222887) and that of A.G. by the Comisión Nacional de Investigación Científica y Tecnológica (Chile)/Basal Financial Project (PFB 12/2007). %< avec comité de lecture %J Molecular Biology of the Cell %I American Society for Cell Biology %V 22 %N 23 %P 4621-34 %8 2011-12 %D 2011 %R 10.1091/mbc.E11-04-0320 %M 21998201 %Z Life Sciences [q-bio]/Microbiology and ParasitologyJournal articles %X Sorting of glycosylphosphatidyl-inositol--anchored proteins (GPI-APs) in polarized epithelial cells is not fully understood. Oligomerization in the Golgi complex has emerged as the crucial event driving apical segregation of GPI-APs in two different kind of epithelial cells, Madin-Darby canine kidney (MDCK) and Fisher rat thyroid (FRT) cells, but whether the mechanism is conserved is unknown. In MDCK cells cholesterol promotes GPI-AP oligomerization, as well as apical sorting of GPI-APs. Here we show that FRT cells lack this cholesterol-driven oligomerization as apical sorting mechanism. In these cells both apical and basolateral GPI-APs display restricted diffusion in the Golgi likely due to a cholesterol-enriched membrane environment. It is striking that N-glycosylation is the critical event for oligomerization and apical sorting of GPI-APs in FRT cells but not in MDCK cells. Our data indicate that at least two mechanisms exist to determine oligomerization in the Golgi leading to apical sorting of GPI-APs. One depends on cholesterol, and the other depends on N-glycosylation and is insensitive to cholesterol addition or depletion. %G English %2 https://pasteur.hal.science/pasteur-00663149/document %2 https://pasteur.hal.science/pasteur-00663149/file/4621.full.pdf %L pasteur-00663149 %U https://pasteur.hal.science/pasteur-00663149 %~ PASTEUR %~ RIIP_PARIS %~ MEMBRANE-TRAFFIC-PATHOGENESIS %~ UMR3691