%0 Journal Article %T Macrophages and myeloid dendritic cells, but not plasmacytoid dendritic cells, produce IL-10 in response to MyD88- and TRIF-dependent TLR signals, and TLR-independent signals. %+ Division of Immunoregulation %+ Schering-Plough Laboratories for Immunological Research %+ Instituto de ciencias da Vida e Saude %+ Department of Host Defense %+ Exploratory Research for Advanced Technology %+ Développement des Lymphocytes %+ Department of immunology %A Boonstra, André %A Rajsbaum, Ricardo %A Holman, Mary %A Marques, Rute %A Asselin-Paturel, Carine %A Pereira, João Pedro %A Bates, Elizabeth E M %A Akira, Shizuo %A Vieira, Paulo %A Liu, Yong-Jun %A Trinchieri, Giorgio %A O'Garra, Anne %< avec comité de lecture %@ 0022-1767 %J Journal of Immunology %I Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists %V 177 %N 11 %P 7551-8 %8 2006-12-01 %D 2006 %R 10.4049/jimmunol.177.11.7551 %M 17114424 %Z Life Sciences [q-bio]/ImmunologyJournal articles %X We have previously reported that mouse plasmacytoid dendritic cells (DC) produce high levels of IL-12p70, whereas bone marrow-derived myeloid DC and splenic DC produce substantially lower levels of this cytokine when activated with the TLR-9 ligand CpG. We now show that in response to CpG stimulation, high levels of IL-10 are secreted by macrophages, intermediate levels by myeloid DC, but no detectable IL-10 is secreted by plasmacytoid DC. MyD88-dependent TLR signals (TLR4, 7, 9 ligation), Toll/IL-1 receptor domain-containing adaptor-dependent TLR signals (TLR3, 4 ligation) as well as non-TLR signals (CD40 ligation) induced macrophages and myeloid DC to produce IL-10 in addition to proinflammatory cytokines. IL-12p70 expression in response to CpG was suppressed by endogenous IL-10 in macrophages, in myeloid DC, and to an even greater extent in splenic CD8alpha(-) and CD8alpha(+) DC. Although plasmacytoid DC did not produce IL-10 upon stimulation, addition of this cytokine exogenously suppressed their production of IL-12, TNF, and IFN-alpha, showing trans but not autocrine regulation of these cytokines by IL-10 in plasmacytoid DC. %G English %L pasteur-00362493 %U https://pasteur.hal.science/pasteur-00362493 %~ PASTEUR %~ RIIP_PARIS