%0 Journal Article %T Reinforcing effects of nicotine microinjections into the ventral tegmental area of mice: dependence on cholinergic nicotinic and dopaminergic D1 receptors. %+ Neurosciences cognitives (NC) %+ Récepteurs et Cognition (RC) %A David, Vincent %A Besson, Morgane %A Changeux, Jean-Pierre %A Granon, Sylvie %A Cazala, Pierre %< avec comité de lecture %@ 0028-3908 %J Neuropharmacology %I Elsevier %V 50 %N 8 %P 1030-40 %8 2006-06 %D 2006 %R 10.1016/j.neuropharm.2006.02.003 %M 16580026 %Z Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/NeurobiologyJournal articles %X We used an intracranial self-administration (ICSA) procedure to assess the involvement of the ventral tegmental area (VTA) nicotinic receptors in the rewarding effects of nicotine. We then challenged intra-VTA nicotine self-administration via systemic or local injections of dopamine (DA)-D1 and nicotinic receptor antagonists. C57BL/6J mice were stereotaxically implanted unilaterally with a guide cannula above the VTA. After 1 week of recovery, mice were allowed to discriminate between two arms of a Y-maze over seven daily sessions, one arm being reinforced by intracranial nicotine microinjection. Mice exhibited nicotine self-administration at both doses tested, i.e. 10 ng (21.6 pmol) and 100 ng (216 pmol)/50-nl injection. In contrast, mice receiving a 216-pmol nicotine dose 0.8 mm above VTA performed at chance level. Once the ICSA response was acquired, systemic pretreatment with the DA-D1 receptor antagonist SCH 23390 (25 microg/kg i.p.) or co-infusion of the nAChR antagonist DHbetaE with nicotine disrupted ICSA. Replacement of SCH 23390 by vehicle, or withdrawal of DHbetaE from nicotine/DHbetaE mixed solutions led to recovery of intra-VTA nicotine self-administration. We conclude that nicotinic receptors in the VTA, presumably alpha4beta2 nAChRs are critically to mediate the rewarding effects of nicotine and that DA-D1 receptors are also directly implicated. %G English %L pasteur-00161455 %U https://pasteur.hal.science/pasteur-00161455 %~ PASTEUR %~ CNRS %~ CDF %~ RIIP_PARIS %~ PSL %~ CDF-PSL