Article Dans Une Revue (Data Paper) Cell Reports Année : 2024

Visualizing the spatial organization of monocytes, interstitial macrophages, and tissue-specific macrophages in situ

Résumé

Tissue-resident mononuclear phagocytes (MPs) are an abundant cell population whose localization in situ reflects their identity. To enable assessment of their heterogeneity, we developed the red/green/blue (RGB)-Mac mouse based upon combinations of Cx3cr1 and Csf1r reporter transgenes, providing a complete visualization of their spatial organization in situ. 3D-multi-photon imaging for spatial mapping and spectral cytometry employing the three markers in combination distinguished tissue-associated monocytes, tissue-specific macrophages, and three subsets of connective-tissue-associated MPs, including CCR2+ monocyte-derived cell, CX3CR1+, and FOLR2+ interstitial subsets, associated with distinct sub-anatomic territories. These populations were selectively reduced by blockade of CSF1, CSF2, CCR2, and CX3CR1 and efficiently reconstitute their spatial distribution after transient myelo-ablation, suggesting an autonomous regulatory environment. Our findings emphasize the organization of the MP compartment at the subanatomic level under steady-state conditions, thereby providing a holistic understanding of their relative heterogeneity across different tissues.
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hal-04889092 , version 1 (15-01-2025)

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Maxime Petit, Eléonore Weber-Delacroix, François Lanthiez, Sandrine Barthélémy, Noëlline Guillou, et al.. Visualizing the spatial organization of monocytes, interstitial macrophages, and tissue-specific macrophages in situ. Cell Reports, 2024, 43 (10), pp.114847. ⟨10.1016/j.celrep.2024.114847⟩. ⟨hal-04889092⟩
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