Tracking the structural dynamics of proteins with time-resolved X-ray solution scattering - Groupe Dynamique et Cinétique des processus moléculaires / Dynamics and Kinetics of Molecular Processes Group (IBS-DYNAMOP)
Article Dans Une Revue Current Opinion in Structural Biology Année : 2023

Tracking the structural dynamics of proteins with time-resolved X-ray solution scattering

Résumé

Relevant events during protein function such as ligand binding/release and interaction with substrates or with light are often accompanied by out-of-equilibrium structural dynamics. Time-resolved experimental techniques have been developed to follow protein structural changes as they happen in real time after a given reaction-triggering event. Time-resolved X-ray solution scattering is a promising approach that bears structural sensitivity with temporal resolution in the femto-to-millisecond time range, depending on the X-ray source characteristics and the triggering method. Here we present the basic principles of the technique together with a description of the most relevant results recently published and a discussion on the computational methods currently developed to achieve a structural interpretation of the time-resolved X-ray solution scattering experimental data.
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Dates et versions

hal-04213310 , version 1 (05-02-2024)

Identifiants

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Kevin Pounot, Giorgio Schirò, Matteo Levantino. Tracking the structural dynamics of proteins with time-resolved X-ray solution scattering. Current Opinion in Structural Biology, 2023, 82, pp.102661. ⟨10.1016/j.sbi.2023.102661⟩. ⟨hal-04213310⟩
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