Rational Design of Photoconvertible and Biphotochromic Fluorescent Proteins for Advanced Microscopy Applications - Groupe Dynamique et Cinétique des processus moléculaires / Dynamics and Kinetics of Molecular Processes Group (IBS-DYNAMOP)
Article Dans Une Revue Chemistry and Biology Année : 2011

Rational Design of Photoconvertible and Biphotochromic Fluorescent Proteins for Advanced Microscopy Applications

Résumé

Advanced fluorescence imaging, including subdiffraction microscopy, relies on fluorophores with controllable emission properties. Chief among these fluorophores are the photoactivatable fluorescent proteins capable of reversible on/off photoswitching or irreversible green-to-red photoconversion. IrisFP was recently reported as the first fluorescent protein combining these two types of phototransformations. The introduction of this protein resulted in new applications such as super-resolution pulse-chase imaging. However, the spectroscopic properties of IrisFP are far from being optimal and its tetrameric organization complicates its use as a fusion tag. Here, we demonstrate how four-state optical highlighting can be rationally introduced into photoconvertible fluorescent proteins and develop and characterize a new set of such enhanced optical highlighters derived from mEosFP and Dendra2. We present in particular NijiFP, a promising new fluorescent protein with photoconvertible and biphotochromic properties that make it ideal for advanced fluorescence-based imaging applications.

Domaines

Biophysique
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Dates et versions

hal-02389699 , version 1 (07-02-2024)

Identifiants

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Virgile Adam, Benjamien Moeyaert, Charlotte c. David, Hideaki Mizuno, Mickaël Lelimousin, et al.. Rational Design of Photoconvertible and Biphotochromic Fluorescent Proteins for Advanced Microscopy Applications. Chemistry and Biology, 2011, 18 (10), pp.1241-1251. ⟨10.1016/j.chembiol.2011.08.007⟩. ⟨hal-02389699⟩
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